The composition of Madagascar's wildlife reflects the fact that the island has been isolated for about 88 million years. The prehistoric breakup of the supercontinent Gondwana separated the Madagascar-Antarctica-India landmass from the Africa-South America landmass around 135 million years ago. Madagascar later split from India about 88 million years ago, allowing plants and animals on the island to evolve in relative isolation.

As a result of the island's long isolation from neighboring continents, Madagascar is home to an abundance of plants and animals found nowhere else on Earth. Approximately 90 percent of all plant and animal species found in Madagascar are endemic, including the lemurs (a type of strepsirrhine primate), the carnivorous fossa and many birds. This distinctive ecology has led some ecologists to refer to Madagascar as the "eighth continent", and the island has been classified by Conservation International as a biodiversity hotspot. As recent as 2021, the "smallest reptile on earth" was also found in Madagascar, known as the Brookesia nana, or nano-chameleon.

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